Product Description
Methylene blue is a reversible redox dyestuff, presenting blue in oxidized status and turning colorless after reduction. With stable chemical activity and mild biological properties, it has long been a core multifunctional reagent integrating biological staining, biochemical testing and clinical treatment.
Product Parameters
| Melting point | 190 °C (dec.)(lit.) |
| Density | 1.0 g/mL at 20 °C |
| Refractive index | n20/D1.347 |
| Fp | 45 °C |
| Storage temp | room temp |
| Solubility | Soluble in water, ethanol, ethylene glycol, methyl cellosolve |
| Form | Liquid |
| Pka | 2.6, 11.2(at 25℃) |
| Colour Index | 52015 |
| color | Green |
| Specific Gravity | 0.98 |
| Odor | Odorless |
| Water Solubility | 40 g/L (20 ºC) |
| λmax | 661 nm |
| Merck | 146060 |
| BRN | 3641570 |
| Biological Applications | Detecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas, erysipelas,hidradenitis suppurativa,inflammation,skin diseases |
| NIST Chemistry Reference | Methylene blue(61-73-4) |
| EPA Substance Registry System | Methylene blue (61-73-4) |
Safety Information
| Hazard Codes | Xn |
| Risk Statements | 10-41-22-20/21/22-36/37/38 |
| Safety Statements | 26-39-36 |
| RIDADR | UN 1993 3/PG 3 |
| WGK Germany | 3 |
| RTECS | SP5740000 |
| HS Code | 32049010 |
| Hazardous Substances Data | 61-73-4(Hazardous Substances Data) |
| Toxicity | LD50 oral in rat: 1180mg/kg |
Application Scenarios
Biological & Microbial Detection – It enables intuitive viability identification of yeast cells: metabolically active cells reduce the dye to colorless, while inactive or dead cells retain blue staining. It is also widely used for bacterial and tissue staining in biological experiments.
Medical Clinical Treatment – Applied for detoxification and symptomatic treatment of multiple poisoning symptoms and specific drug-induced neurological complications.
Industrial Coloring Formulation – Suitable for dyeing silk, linen, paper, bamboo and wood materials. It can be formulated into printing inks and lake pigments, and compounded with other colorants to prepare customized royal blue tones for industrial coloring.
